Effects of Cryoprotectants in Combination on the Survival of Frozen Sugarcane Cells

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Effects of cryoprotectants in combination on the survival of frozen sugarcane cells.

Cryoprotection of suspension cultures of sugarcane cells (Saccharum sp.) during freezing to various temperatures was tested using glucose, dimethylsulfoxide, and ethylene glycol at various concentrations, alone and in combinations. Viability of the thawed cells was assessed by triphenyltetrazolium chloride reduction, cell growth, and microscopic examination. Enhanced cryoprotection-as much as a...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1979

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.63.4.598